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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Development of a promising microbial platform for the production of dicarboxylic acids from biorenewable resources

Fig. 3

Schematic representation of the expression cassettes and chromosome integration in W. sorbophila. The pUC18-nat1 (a) and pUC18-ble (b) cassettes, each containing the TEF promoter and GAPDH terminator, were PCR amplified using primers TEFp-F and GAPt-R and randomly inserted into the chromosome. The gel images show the results of yeast colony PCR using primers TEFp-F and GAPt-R. The sizes of the bands are 1.4 kb (a) and 1.2 kb (b)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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