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Table 2 HR efficiency depending on the growth stage

From: Development of a promising microbial platform for the production of dicarboxylic acids from biorenewable resources

Growth stage

Strain

Target gene

Marker gene

HR efficiency

Early stationary phase

DS02

ADE2

ble

0% (1365)a

DS02

URA3

ble

0% (186)a

UHP1

POX1

URA3

56.3% (48)a

UHP2

POX2

URA3

41.7% (48)a

Log phase

DS02

ADE2

ble

1% (1470)a

DS02

URA3

ble

0% (186)a

UHP1

POX1

URA3

81.3% (48)a

UHP2

POX2

URA3

83.3 (48)a

S arrest

DS02

ADE2

ble

25.6% (379)a

DS02

URA3

ble

4.8% (186)a

UHP1

POX1

URA3

97.9% (48)a

UHP2

POX2

URA3

97.9% (48)a

  1. aNumber of transformants screened