Rewiring glycerol metabolism for enhanced production of poly-γ-glutamic acid in Bacillus licheniformis

Table 1 Comparison of γ-PGA fermentation between WX-02 and mutant strains

B. licheniformis strains	Biomass (OD₆₀₀)	Glycerol consumption (g/L)	γ-PGA titer (g/L)
WX-02	10.99 ± 0.34	30.51 ± 0.98	12.83 ± 0.27
BC2	12.39 ± 0.65	37.17 ± 0.85	17.42 ± 1.17
BC3	13.35 ± 0.32	38.95 ± 0.92	18.85 ± 0.53
BC4	13.33 ± 0.69	40.19 ± 0.73	19.20 ± 1.57

Strains were grown in 250-mL flasks containing 50-mL medium and incubated in a rotary shaker with 230 rpm at 37 °C for 48 h. The initial glycerol concentration was 60 g/L. Data are presented as mean ± SDs of three replicates

ISSN: 2731-3654