Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

Table 3 Comparison of K. pneumoniae parent strain (kpG), overexpressing strains, and deleting strains in a 72-h flask cultivation; the values given represent mean ± SD

	Strains	Biomass (g/L)	Consumption of xylose (g/L)	2,3-BD concentration (g/L)	Utilization ratio of xylose (%)	2,3-BD yield (g/g)
	kpG	3.57 ± 0.05	45.49 ± 0.69	12.78 ± 0.13	37.9 ± 0.69	0.28 ± 0.01
Pentose phosphate pathway	kp-araB	3.72 ± 0.03	49.4 ± 0.87	14.22 ± 0.19	41.2 ± 0.72	0.29 ± 0.02
Pentose phosphate pathway	kp-tktA	3.89 ± 0.03	55.22 ± 0.98	18.03 ± 0.15	46.0 ± 0.86	0.33 ± 0.05
Signal transduction and electronic transmission	kp-pntA	3.84 ± 0.02	50.72 ± 0.69	16.38 ± 0.22	42.3 ± 0.58	0.32 ± 0.02
Signal transduction and electronic transmission	kp-nuoF	3.88 ± 0.07	54.77 ± 0.78	16.15 ± 0.12	45.6 ± 0.55	0.29 ± 0.04
Glycolysis	kp-gapA	3.91 ± 0.04	57.5 ± 1.01	14.34 ± 0.11	47.9 ± 0.72	0.25 ± 0.04
Transmembrane transport	kp-ppk	3.88 ± 0.05	54.86 ± 0.97	15.81 ± 0.07	45.7 ± 0.83	0.29 ± 0.01
Transmembrane transport	kp-xylG	3.69 ± 0.06	46.54 ± 0.99	13.03 ± 0.14	38.8 ± 0.56	0.3 ± 0.02
The synthesis of byproduct	ΔadhE	3.86 ± 0.03	52.27 ± 0.86	14.12 ± 0.12	43.6 ± 0.63	0.27 ± 0.03
The synthesis of byproduct	ΔsfcA	3.59 ± 0.03	45.58 ± 0.66	13.09 ± 0.11	40.0 ± 0.58	0.29 ± 0.03

ISSN: 2731-3654