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Table 3 Comparison of K. pneumoniae parent strain (kpG), overexpressing strains, and deleting strains in a 72-h flask cultivation; the values given represent mean ± SD

From: Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

  Strains Biomass (g/L) Consumption of xylose (g/L) 2,3-BD concentration (g/L) Utilization ratio of xylose (%) 2,3-BD yield (g/g)
  kpG 3.57 ± 0.05 45.49 ± 0.69 12.78 ± 0.13 37.9 ± 0.69 0.28 ± 0.01
Pentose phosphate pathway kp-araB 3.72 ± 0.03 49.4 ± 0.87 14.22 ± 0.19 41.2 ± 0.72 0.29 ± 0.02
kp-tktA 3.89 ± 0.03 55.22 ± 0.98 18.03 ± 0.15 46.0 ± 0.86 0.33 ± 0.05
Signal transduction and electronic transmission kp-pntA 3.84 ± 0.02 50.72 ± 0.69 16.38 ± 0.22 42.3 ± 0.58 0.32 ± 0.02
kp-nuoF 3.88 ± 0.07 54.77 ± 0.78 16.15 ± 0.12 45.6 ± 0.55 0.29 ± 0.04
Glycolysis kp-gapA 3.91 ± 0.04 57.5 ± 1.01 14.34 ± 0.11 47.9 ± 0.72 0.25 ± 0.04
Transmembrane transport kp-ppk 3.88 ± 0.05 54.86 ± 0.97 15.81 ± 0.07 45.7 ± 0.83 0.29 ± 0.01
kp-xylG 3.69 ± 0.06 46.54 ± 0.99 13.03 ± 0.14 38.8 ± 0.56 0.3 ± 0.02
The synthesis of byproduct ΔadhE 3.86 ± 0.03 52.27 ± 0.86 14.12 ± 0.12 43.6 ± 0.63 0.27 ± 0.03
ΔsfcA 3.59 ± 0.03 45.58 ± 0.66 13.09 ± 0.11 40.0 ± 0.58 0.29 ± 0.03