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Table 4 Strains and plasmids used in this study

From: Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

Strains or plasmids Relevant properties Reference or source
Strains
 E. coli DH5α supE44 ΔlacU169 (ϕ 80 lacZΔM15) hsdR17 recAl endAl gyrA96 thi-1 relA TaKaRa
 E. coliS17-1λpir TpR SmR recA thi-1 pro hsdR-M+RP4:2-Tc:Mu: Km Tn7 λpir [50]
 kpG Klebsiella pneumonia CICC 10781 [51]
 kpC kpG directed evolution This study
 kp-araB kpG containing plasmid pUC18k- araB This study
 kp-tktA kpG containing plasmid pUC18k-tktA This study
 kp-pntA kpG containing plasmid pUC18k-pntA This study
 kp-gapA kpG containing plasmid pUC18k-gapA This study
 kp-nuoF kpG containing plasmid pUC18k-nuoF This study
 kp-ppk kpG containing plasmid pUC18k-ppk This study
 kp-xylG kpG containing plasmid pUC18k-xylG This study
 ΔadhE An alcohol dehydrogenase gene-deficient mutant of kpG This study
 ΔsfcA An malate dehydrogenase gene-deficient mutant of kpG This study
Plasmids
 pUC18k Kamr, pUC ori, Plac, MCS Lab archive
 pDK7 Cmr Lab archive
 pUC18k-araB Kamr, pUC18k containing araB gene This study
 pUC18k-xylG Kamr, pUC18k containing xylG gene This study
 pUC18k-tktA Kamr, pUC18k containing tktA gene This study
 pUC18k-pntA Kamr, pUC18k containing pntA gene This study
 pUC18k-gapA Kamr, pUC18k containing gapA gene This study
 pUC18k-nuoF Kamr, pUC18k containing nuoF gene This study
 pUC18k-ppk Kamr, pUC18k containing ppk gene This study
 pJTOOL-3 Suicide vector hphr SacB oriT Lab archive
 pMQ300 hphr Lab archive
 pFLP2 Ampr encodes FLP recombinase Lab archive
 p-adhE Hphr, pJTOOL-3 derivative, where a adhE::HphFRT cassettes was inserted This study
 p-sfcA Hphr, pJTOOL-3 derivative, where a sfcA::HphFRT cassettes was inserted This study