Table 4 Strains and plasmids used in this study
Strains or plasmids | Relevant properties | Reference or source |
|---|---|---|
Strains | ||
 E. coli DH5α | supE44 ΔlacU169 (ϕ 80 lacZΔM15) hsdR17 recAl endAl gyrA96 thi-1 relA | TaKaRa |
 E. coliS17-1λpir | TpR SmR recA thi-1 pro hsdR-M+RP4:2-Tc:Mu: Km Tn7 λpir | [50] |
 kpG | Klebsiella pneumonia CICC 10781 | [51] |
 kpC | kpG directed evolution | This study |
 kp-araB | kpG containing plasmid pUC18k- araB | This study |
 kp-tktA | kpG containing plasmid pUC18k-tktA | This study |
 kp-pntA | kpG containing plasmid pUC18k-pntA | This study |
 kp-gapA | kpG containing plasmid pUC18k-gapA | This study |
 kp-nuoF | kpG containing plasmid pUC18k-nuoF | This study |
 kp-ppk | kpG containing plasmid pUC18k-ppk | This study |
 kp-xylG | kpG containing plasmid pUC18k-xylG | This study |
 ΔadhE | An alcohol dehydrogenase gene-deficient mutant of kpG | This study |
 ΔsfcA | An malate dehydrogenase gene-deficient mutant of kpG | This study |
Plasmids | ||
 pUC18k | Kamr, pUC ori, Plac, MCS | Lab archive |
 pDK7 | Cmr | Lab archive |
 pUC18k-araB | Kamr, pUC18k containing araB gene | This study |
 pUC18k-xylG | Kamr, pUC18k containing xylG gene | This study |
 pUC18k-tktA | Kamr, pUC18k containing tktA gene | This study |
 pUC18k-pntA | Kamr, pUC18k containing pntA gene | This study |
 pUC18k-gapA | Kamr, pUC18k containing gapA gene | This study |
 pUC18k-nuoF | Kamr, pUC18k containing nuoF gene | This study |
 pUC18k-ppk | Kamr, pUC18k containing ppk gene | This study |
 pJTOOL-3 | Suicide vector hphr SacB oriT | Lab archive |
 pMQ300 | hphr | Lab archive |
 pFLP2 | Ampr encodes FLP recombinase | Lab archive |
 p-adhE | Hphr, pJTOOL-3 derivative, where a adhE::HphFRT cassettes was inserted | This study |
 p-sfcA | Hphr, pJTOOL-3 derivative, where a sfcA::HphFRT cassettes was inserted | This study |