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Table 2 Top 30 extracellular proteins in C. acetobutylicum biofilm identified by LC–MS/MS

From: Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins

  Gene locus Scorea Massb Matchesc Sequencesd emPAIe Description
1 CA_C2703 10,961 58,166 408 29 12.3 Molecular chaperone GroEL (Hsp60)
2 CEA_G3563 3556 47,277 139 17 10.3 Putative S-layer protein
3 CA_C3597 8141 20,493 187 10 10.3 Rubrerythrin
4 CA_C2710 2299 28,089 71 14 9.5 Electron transfer flavoprotein beta-subunit
5 CA_C0709 1619 35,999 72 15 8.1 Glyceraldehyde-3-phosphate dehydrogenase
6 CA_C2452 572 15,611 24 7 7.7 Flavodoxin
7 CA_C1555 2446 29,503 80 10 7.5 Flagellin
8 CA_C1747 556 8602 21 3 6.9 Acyl carrier protein, ACP
9 CA_C3136 5404 43,482 207 18 6.8 Elongation Factor Tu (Ef-Tu)
10 CA_C2990 396 7307 23 2 6.2 Cold shock protein
11 CA_C1834 448 9203 19 4 5.9 Host factor I protein Hfq
12 CA_C3125 299 7908 7 3 5.4 Ribosomal protein L29
13 CA_C2712 571 28,400 28 10 4.9 Crotonase
14 CA_C1807 182 10,251 7 5 4.8 Ribosomal Protein S15
15 CA_C3211 1113 10,341 59 6 4.7 DNA binding protein HU
16 CA_P0164 1098 23,666 33 8 4.6 Acetoacetyl-CoA:acetate/butyrate CoA-transferase subunit B
17 CA_C2704 745 10,420 27 5 4.6 Molecular chaperone groES (Hsp10, Hsp60 cofactor)
18 CA_C3145 553 12,670 24 5 4.4 Ribosomal protein L7/L12
19 CA_C3076 627 32,321 30 12 4.3 Phosphate butyryltransferase
20 CA_C1281 757 17,734 29 7 3.8 Heat shock protein grpE (hsp20, Hsp70 cofactor)
21 CA_C1282 2280 65,723 77 24 3.8 Molecular chaperone DnaK (Hsp70)
22 CA_C2229 4951 129,740 191 43 3.7 Pyruvate:ferredoxin oxidoreductase
23 CA_C2641 782 49,565 37 19 3.7 FKBP-type peptidyl-prolyl cis-transisomerase (trigger factor)
24 CA_C3075 744 39,146 33 15 3.7 Butyrate kinase, BUK
25 CA_C2873 1247 41,443 53 16 3.7 Acetyl coenzyme A acetyltransferase (thiolase)
26 CA_P0165 725 23,797 22 8 3.3 Acetoacetate decarboxylase
27 CA_P0162 3768 95,774 175 32 3.2 Alcohol dehydrogenase E
28 CA_C0711 452 26,698 17 11 3.1 Triosephosphate isomerase
29 CA_C2597 461 17,599 19 5 3.1 Hypothetical protein
30 CA_C3558 1128 48,599 51 10 3.0 Probable S-layer protein
  1. aAll scores were statistically significant (p < 0.05; Student t test). Higher score means higher probability
  2. bTheoretical molecular mass
  3. cThe number of peptides that matched the identified protein with p < 0.05
  4. dThe number of distinct (nonredundant) peptides that matched the identified protein with p < 0.05 (Student t test)
  5. eExponentially modified Protein Abundance Index