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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in Phaeodactylum tricornutum

Fig. 1

Molecular characteristics of GPAT1 and LPAT1 co-overexpressed lines (OE1 and OE2) and wild-type (WT). a Relative expression abundance of GPAT1 during the whole growth period (13 days), β-actin was served as an internal reference gene; b relative expression abundance of LPAT1 during the whole growth period, β-actin was served as an internal reference gene; c Western blot analysis for detection of c-Myc and flag. Cells were collected on day 7. Lane 1 and 2 represent OE1 and OE2 lines, respectively. β-actin was served as a housekeeping protein; d changes of GPAT1 enzymatic activity during the whole growth phase; e changes of LPAT1 enzymatic activity in microalgae during the whole growth phase. A significant difference between WT and OE lines is indicated at the p < 0.05 (*) or p < 0.01 (**) level. Each value represents the mean ± SD (n = 3)

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