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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in Phaeodactylum tricornutum

Fig. 3

Primary metabolites and relative neutral lipid content of overexpression lines (OE1 and OE2) and wild-type (WT). a Total carbohydrate content of cells harvested on day 4 and 7; b soluble protein content of cells collected on day 4 and 7. c relative neutral lipid content of GPAT1 and LPAT1 overexpression lines (OE) using Nile-red staining method under normal nutrient condition during the whole growth period (13 days); d relative neutral lipid content in cells under normal condition (Normal) and cells subjected to 48 h of N (-N) or P (-P) deprivation. Cells were harvested on day 7 and subsequently placed into nutrient-replete and depleted media for 48 h of culture, respectively. A significant difference between WT and OE lines is indicated at the p < 0.05 (*) or p < 0.01 (**) level. Each value represents the mean ± SD (n = 3)

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