Fig. 3From: Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in Phaeodactylum tricornutumPrimary metabolites and relative neutral lipid content of overexpression lines (OE1 and OE2) and wild-type (WT). a Total carbohydrate content of cells harvested on day 4 and 7; b soluble protein content of cells collected on day 4 and 7. c relative neutral lipid content of GPAT1 and LPAT1 overexpression lines (OE) using Nile-red staining method under normal nutrient condition during the whole growth period (13 days); d relative neutral lipid content in cells under normal condition (Normal) and cells subjected to 48 h of N (-N) or P (-P) deprivation. Cells were harvested on day 7 and subsequently placed into nutrient-replete and depleted media for 48 h of culture, respectively. A significant difference between WT and OE lines is indicated at the p < 0.05 (*) or p < 0.01 (**) level. Each value represents the mean ± SD (n = 3)Back to article page