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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Real-time adsorption and action of expansin on cellulose

Fig. 3

Transition model for BsEXLX1 adsorption onto cellulose. a Binding/washoff profiles of BsEXLX1 at different concentrations (25 °C, 100 μl/min). Protein concentrations from bottom to top are 5, 10, 25, 50, and 100 ppm. b Maximum and irreversible binding of 50 ppm BsEXLX1 with different injecting times. c Initial rate of BsEXLX1 adsorption to cellulose. The slope of the fit line is equal to the kinetic parameter kA·Γmax = 0.2375 ng/(cm2 s ppm). R2 = 0.99. d The kinetic parameter Γmax was determined from the adsorption of increasing concentrations of BsEXLX1 to the surface. The maximum surface concentration is shown (Bullet) vs. bulk expansion concentration, and reaches a near-maximal value of 163.53 ng/cm2. e Experimental binding isotherms at [E]bulk = 50 ppm (red) compared to the prediction model (black)

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