Fig. 2
Improving fumarate production by overexpression of fumarase genes. a Series of gene expression cassettes with various fumarase genes under control of strong constitutive promoter. b Fumarate production in shake-flask culture of mutants overexpressing various fumarase genes. c Specific enzyme activities of fumarase (fumarate to malate) in transformants. Enzymatic activity of fumarase in strain SG103 served as the control. Data are average of three replicates with standard error