Cellulose-specific Type B carbohydrate binding modules: understanding oligomeric and non-crystalline substrate recognition mechanisms

Table 1 Binding affinities of a given family 4, 17, or 28 CBM for cellopentaose

References	CBM	Method	Temperature (K)	pH	Buffer conditions	∆G° (kcal/mol)
Tomme et al. [23]	CfCBM4-1	ITC	308	7.0	Pure water	− 5.23 ± 0.91
Tomme et al. [23]	CfCBM4-1	ITC	308	7.0	50 mM potassium phosphate	− 6.09 ± 0.64
Tomme et al. [23]	CfCBM4-1	ITC	308	7.0	50 mM potassium phosphate 1.0 M NaCl	− 6.21 ± 0.57
Brun et al. [58]	CfCBM4-2	ITC	308	7.0	50 mM potassium phosphate 1.0 M NaCl	− 5.80 ± 0.005
Boraston et al. [22]	CcCBM17	Fluorescence	298	7.5	25 mM Tris–HCl	− 6.72
Boraston et al. [22]	CcCBM17	Fluorescence	298	7.5	25 mM Tris–HCl 0.5 M NaCl	− 6.93
Boraston et al. [22]	CcCBM17	Fluorescence	298	7.5	25 mM Tris–HCl 1.0 M NaCl	− 7.05
Boraston et al. [22]	CcCBM17	Fluorescence	298	7.5	25 mM Tris–HCl 2.0 M NaCl	− 7.24
Notenboom et al. [24]	CcCBM17	ITC	298	7.0	50 mM potassium phosphate	− 5.80 ± 0.03
Boraston et al. [21]	Bsp CBM28	UV	298	7.0	25 mM Tris–HCl	− 5.81 ± 0.14
Boraston et al. [21]	Bsp CBM28	ITC	298	7.0	50 mM potassium phosphate	− 5.93 ± 0.003
Araki et al. [29]	CjCBM17	ITC	293	7.0	100 mM potassium phosphate	− 7.7 ± 0.3
Araki et al. [29]	CjCBM28	ITC	293	7.0	100 mM potassium phosphate	− 7.7 ± 0.6

The CBMs listed here include: Cellulomonas fimi CBM4-1 and CBM4-2 (CfCBM4-1 and CfCBM4-2), Clostridium cellulovorans CBM17 (CcCBM17), Bacillus sp. 1139 CBM28 (BspCBM28), and Clostridium josui CBM28 (CjCBM28). The methods for obtaining binding affinities in the referenced study are listed as either isothermal titration calorimetry (ITC), fluorescence emission scans, or ultraviolet (UV) difference spectra. Reported experimental conditions are as given

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