Fig. 6

Cellobiose fermentation of E. coli BL21(pET-celo1), E. coli BL21(pET-celo2) and E. coli BL21(pETDuet-1). a Time courses of cellobiose consumption. b Time course of optical density. Purple, E. coli BL21(pET-celo1); Blue, E. coli BL21(pET-celo2); Olive, E. coli BL21(pETDuet-1). Fermentations were carried out in 100-mL Erlenmeyer flasks containing 30-mL LB medium in the presence of 10 g/L cellobiose, 100 mg/L ampicillin, and 0.2 mM IPTG at 37 °C and 200 rpm on a rotary shaker