Fig. 3

Effect of pH on the repolymerization of the released phenolic product from degradation of dimeric lignin (a) and conversion of the model dimeric lignin by native and variant A55R/N156E-H239E at pH 2.5 (b). The oxidation reaction was carried out in 0.1 M BR buffer, pH 2.0 to 4.5 with 4 mM lignin dimer and 5 μM native LiPH8 or variants, in which H₂O₂ was fed at a rate of 150 μM/15 min at 25 °C