In silico-designed lignin peroxidase from Phanerochaete chrysosporium shows enhanced acid stability for depolymerization of lignin

Table 2 Stability of LiPH8 variants under acidic pH conditions

LiPH8 variant	Half-life time t_1/2 (min)	Change of Gibbs free energy ΔΔG_mut (kcal/mol)
Native LiPH8	9.9 ± 0.8
LiPH8 variants with in silico prediction as stabilizing effect^a
A16E	70.6 ± 4.7	− 0.99
A55R/N156E	21.5 ± 3.5	− 0.58
H239E	117.7 ± 24.5	− 3.58
A16E-A55R/N156E	19.7 ± 0.4	− 0.68
A16E-H239E	47.8 ± 3.7	− 2.41
A55R/N156E-H239E	113.54 ± 17.2	− 1.46
A16E-A55R/N156E-H239E	53.0 ± 5.8	− 2.17
LiPH8 variants with in silico prediction as neutral/destabilizing effect^a
A36E/A180K	6.8 ± 1.0	0.22
Q189D	4.5 ± 0.6	0.95
L328D/L299K	13.0 ± 3.7	2.38

^a Effect of mutation is defined as a stabilizing effect, neutral effect and destabilizing effect when the Gibbs free energy change upon mutation, ΔΔG_mut (kcal/mol), is less than − 0.5, from − 0.5 to 0.5 and higher than 0.5, respectively

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