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Table 2 Stability of LiPH8 variants under acidic pH conditions

From: In silico-designed lignin peroxidase from Phanerochaete chrysosporium shows enhanced acid stability for depolymerization of lignin

LiPH8 variant Half-life time t1/2 (min) Change of Gibbs free energy ΔΔGmut (kcal/mol)
Native LiPH8 9.9 ± 0.8  
LiPH8 variants with in silico prediction as stabilizing effecta
 A16E 70.6 ± 4.7 − 0.99
 A55R/N156E 21.5 ± 3.5 − 0.58
 H239E 117.7 ± 24.5 − 3.58
 A16E-A55R/N156E 19.7 ± 0.4 − 0.68
 A16E-H239E 47.8 ± 3.7 − 2.41
 A55R/N156E-H239E 113.54 ± 17.2 − 1.46
 A16E-A55R/N156E-H239E 53.0 ± 5.8 − 2.17
LiPH8 variants with in silico prediction as neutral/destabilizing effecta
 A36E/A180K 6.8 ± 1.0 0.22
 Q189D 4.5 ± 0.6 0.95
 L328D/L299K 13.0 ± 3.7 2.38
  1. a Effect of mutation is defined as a stabilizing effect, neutral effect and destabilizing effect when the Gibbs free energy change upon mutation, ΔΔGmut (kcal/mol), is less than − 0.5, from − 0.5 to 0.5 and higher than 0.5, respectively