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Table 3 Kinetic parameters for oxidation of veratryl alcohol by native enzyme and variants at pH 2.5

From: In silico-designed lignin peroxidase from Phanerochaete chrysosporium shows enhanced acid stability for depolymerization of lignin

Variant KM (μM) kcat (s−1) kcat/KM (s−1 mM−1)
Native LiPH8 121.0 ± 7.9 10.0 ± 0.4 82.8 ± 10.3
LiPH8 variants with the introduction of a single salt bridge
 A16E 57.2 ± 5.9 3.4 ± 0.5 58.6 ± 2.7
 A55R/N156E 141.1 ± 33.6 12.6 ± 0.8 92.4 ± 27.8
 H239E 90.6 ± 11.4 4.9 ± 0.4 54.5 ± 10.8
LiPH8 variants with the introduction of combined salt bridge
 A16E-A55R/N156E 45.4 ± 9.5 5.5 ± 0.1 123.5 ± 25.0
 A16E-H239E 53.4 ± 3.4 7.8 ± 0.0 145.5 ± 8.9
A55R/N156E-H239E 59.8 ± 0.9 9.4 ± 0.1 157.5 ± 2.6
 A16E-A55R/N156E-H239E 56.8 ± 1.6 7.6 ± 0.1 133.3 ± 3.7