Table 3 Kinetic parameters for oxidation of veratryl alcohol by native enzyme and variants at pH 2.5
Variant | KM (μM) | kcat (s−1) | kcat/KM (s−1 mM−1) |
|---|---|---|---|
Native LiPH8 | 121.0 ± 7.9 | 10.0 ± 0.4 | 82.8 ± 10.3 |
LiPH8 variants with the introduction of a single salt bridge | |||
 A16E | 57.2 ± 5.9 | 3.4 ± 0.5 | 58.6 ± 2.7 |
 A55R/N156E | 141.1 ± 33.6 | 12.6 ± 0.8 | 92.4 ± 27.8 |
 H239E | 90.6 ± 11.4 | 4.9 ± 0.4 | 54.5 ± 10.8 |
LiPH8 variants with the introduction of combined salt bridge | |||
 A16E-A55R/N156E | 45.4 ± 9.5 | 5.5 ± 0.1 | 123.5 ± 25.0 |
 A16E-H239E | 53.4 ± 3.4 | 7.8 ± 0.0 | 145.5 ± 8.9 |
A55R/N156E-H239E | 59.8 ± 0.9 | 9.4 ± 0.1 | 157.5 ± 2.6 |
 A16E-A55R/N156E-H239E | 56.8 ± 1.6 | 7.6 ± 0.1 | 133.3 ± 3.7 |