Fig. 2

The gene expression analysis for the selected genes showing differential regulation in Camelina transgenic lines. Data are the fold changes (FC) in expression measured by using both RNA-Seq and qRT-PCR techniques (a, b) in both DGAT1 and GPD1, respectively, relative to WT. The fold change values used in the analysis are presented in Additional file 1: Table S15. Data shown in c, d indicate the relative gene expression for the selected genes measured by qRT-PCR in both DGAT1 and GPD1 lines, respectively, relative to WT. The genes shown here are non-specific lipid transfer 4-like (NSLT-L), glycerol-3-phosphate sn-2-acyltransferase 1 (GPAT1), oleosin 5 (OLE5), 3-ketoacyl-synthase 18-like (KCS18), TAG-lipase 2-like (TAGL2-L), acyl CoA thioesterase 13-like (ACOT13-L), cruciferin 3 (CRU3), acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), oleosin 1 (OLE1), glycerol-3-phosphate acyltransferase 9 (GPAT9), lysophosphatidyl acyltransferase 2 (LPAT2), glycerol-3-phosphate transporter 1 (GLPT1), lysophosphatidyl acyltransferase 5 (LPAT5), glucose-6-phosphate l-epimerase (G6Pe), diacylglycerol kinase 3-like isoform X1 (DAGK), 3-keto acyl-synthase 6 (KCS6), acyl-activating enzyme 7 (Acylae7), glycerol-3-phosphate acyltransferase 5 (GPAT5)