Fig. 5

Alterations of selected metabolite levels in Camelina transgenics relative to WT seeds during seed development. Metabolites levels were determined and the relative peak areas were shown in developing seeds at 10–16, 18–26, and 28–36 DAF of wildtype (WT) and GPD1, DGAT1, and D + G lines overexpressing both ScGPD1 and AtDGAT1 genes. The levels of selected metabolites involved in glycolysis, TCA cycle, acetyl-CoA production, fatty acid synthesis, and TAG assembly are shown in each genotype and in three stages of seed development. The Y-axis represents the median scaled imputed data for the peak raw ion counts for each metabolite. The bars represent box plots where the mean, median, upper and lower quartiles, and the max and min distribution of values are presented. *Significant difference at P < 0.05 compared with the WT, based on Two-way ANOVA test