Skip to main content
Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase

Fig. 6

Working model for the alterations in metabolite profiling in the transgenics relative to WT seeds. The relative metabolite ratios in GPD1, DGAT1, and D + G lines as compared to WT are shown. a The impact of the transgenes on the metabolites involved in glycolysis, TCA cycle, fatty acid synthesis and TAG assembly and degradation, including monoacylglycerols and lysophospholipids are highlighted. b The impact of the transgenes on the monoacylglycerols (MAGs), fatty acids (FAs) and glycerophospholipids are highlighted. Statistical significance of the relative metabolite contents is indicated with different colors. WT, wildtype, GPD1, lines overexpressing ScGPD1 gene, DGAT1, lines overexpressing AtDGAT1 gene, and D + G, lines overexpressing both ScGPD1 and AtDGAT1 genes. The abbreviated metabolites shown are Glc-6P glucose 6-phosphate, Fru-6P fructose 6-phosphate, DHAP dihydroxyacetone phosphate, LPA lysophosphatidic acid, PA phosphatidic acid, PC phosphatidylcholine, LPC 2-lysophosphatidylcholine, DAG diacylglycerol, TAG triacylglycerol, MAG monoacylglycerol, FA fatty acids; C16:0 palmitic acid, C18:0 stearic acid, C18:1 oleic acid, C18:2 linoleic acid, C18:3 α-linolenic acid, C20:1 gondoic acid, C22:1 erucic acid, 1-lyso-PC (16:0) lyso-phosphatidylcholine with 16:0 at sn-1 position (1-palmitoyllysophosphatidylcholine), 1-lyso-PC (18:0) lyso-phosphatidylcholine with 18:0 at sn-1 position (1-stearoyl lyso-phosphocholine), 2-lyso-PC (16:0) lyso-phosphatidylcholine with 16:0 at sn-2 position (2-palmitoylglycerophosphocholine), GPC glycerophosphorylcholine, 1-lyso-PE (16:0) lyso-phosphatidylethanolamine with 16:0 at sn-1 position (1-lysophosphatidylethanolamine), 1-lyso-PE (18:2) lyso-phosphatidylethanolamine with 18:2 at sn-1 position (1-linoleoylglycerophosphoethanolamine), 1-lyso-PI (16:0) lyso-phosphatidylinositol with 16:0 at sn-1 position (1-palmitoylglycerophosphoinositol), 1-lyso-PI (18:1) lyso-phosphatidylinositol with 18:1 at sn-1 position (1-oleoylglycerophosphoinositol), 1-LPA (16:0) 1-palmitoylglycerophosphoglycerol. The abbreviated enzymes shown are NHO1 glycerol kinase, GPAT glycerol 3-phosphate acyltransferase, LPAT lysophospholipids acyltransferase, PAP Phosphatidate phosphatase, LPCAT lysophosphatidylcholine acyltransferase, DHAK dihydroxyacetone kinase, SDP1 triacylglycerol lipase, DGAT1 diacylglycerol acyltransferase 1, PDCT phosphatidylcholine: diacylglycerol cholinephosphotransferase, CPT CDP-choline: diacylglycerol cholinephosphotransferase, MGAT monoacylglycerol acyltransferase, PDH pyruvate dehydrogenase, MDH malate dehydrogenase, ME Malic enzyme

Back to article page