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Table 1 Functional classifications of the DEGs in Camelina transgenics as compared to WT

From: Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase

GO ID

GO term

Counts

Up in DG-14

Down in DG-14

Up in DG-21

Down in DG-21

Up in GP-14

Down in GP-14

Up in GP-21

Down in GP-21

GO:0005488

Binding

80

236

100

152

159

198

178

211

GO:0003824

Catalytic activity

68

282

85

121

130

200

158

173

GO:1901363

Heterocyclic compound binding

24

145

57

91

93

108

116

126

GO:0016787

Hydrolase activity

21

89

30

31

42

57

61

49

GO:0016788

Hydrolase activity, acting on ester bonds

4

29

11

8

13

23

20

12

GO:0043167

Ion binding

52

149

54

87

92

119

104

84

GO:0016874

Ligase activity

4

10

2

3

2

10

1

7

GO:0008289

Lipid binding

4

8

4

1

3

8

7

4

GO:0003676

Nucleic acid binding

24

54

27

55

49

50

48

59

GO:0001071

Nucleic acid binding transcription factor activity

14

29

8

20

24

28

18

30

GO:0097159

Organic cyclic compound binding

24

145

57

91

93

108

116

126

GO:0016491

Oxidoreductase activity

16

81

15

35

31

59

31

42

GO:0005515

Protein binding

2

37

20

20

24

37

34

47

GO:0016740

Transferase activity

28

86

35

50

55

56

70

70

GO:0016746

Transferase activity, transferring acyl groups

4

15

6

6

9

9

9

12

GO:0005215

Transporter activity

12

28

14

10

18

20

22

13

GO:0016209

Antioxidant activity

–

12

–

1

2

8

–

–

GO:0030246

Carbohydrate binding

–

6

2

6

4

5

1

6

GO:0097367

Carbohydrate derivative binding

–

46

21

22

30

31

44

31

GO:0050662

Coenzyme binding

–

5

4

5

2

5

7

9

GO:0048037

Cofactor binding

–

7

6

6

5

7

9

11

GO:0009055

Electron carrier activity

–

5

1

4

3

4

–

3

GO:0016301

Kinase activity

13

27

13

12

16

17

20

18

GO:0016829

Lyase activity

1

19

2

3

3

14

6

2

GO:0046872

Metal ion binding

–

98

30

59

60

88

61

82

GO:0045735

Nutrient reservoir activity

–

22

–

1

–

17

1

1

GO:0000166

Nucleotide binding

–

53

26

31

36

41

58

43

GO:0019825

Oxygen binding

–

14

–

2

3

8

5

3

GO:0005524

ATP binding

–

44

20

19

25

27

34

28

GO:0016887

ATPase activity

7

14

4

4

7

4

9

5

GO:0008233

Peptidase activity

5

17

6

7

5

8

6

10

GO:0042578

Phosphoric ester hydrolase activity

2

9

4

4

7

12

7

5

GO:0016157

Sucrose synthase activity

–

1

–

–

–

1

–

–

GO:0046524

Sucrose-phosphate synthase activity

–

1

–

–

–

–

–

–

GO:0016298

Lipase activity

–

6

2

–

2

3

3

1

GO:0004806

Triglyceride lipase activity

–

–

2

–

1

–

1

–

GO:0005319

Lipid transporter activity

–

1

1

–

–

–

–

–

GO:0047617

Acyl-CoA hydrolase activity

–

–

–

–

–

–

–

1

GO:0016411

Acylglycerol O-acyltransferase activity

–

1

–

1

–

–

3

–

GO:0016421

CoA carboxylase activity

–

–

–

–

–

–

 

1

GO:0047734

CDP-glycerol diphosphatase activity

–

–

1

–

–

–

–

–

GO:0004144

Diacylglycerol O-acyltransferase activity

–

1

–

1

–

–

2

–

GO:0090447

Glycerol-3-phosphate 2-O-acyltransferase activity

–

–

–

–

–

–

1

1

GO:0004366

Glycerol-3-phosphate O-acyltransferase activity

–

–

–

–

–

–

1

1

GO:0052722

Fatty acid in-chain hydroxylase activity

–

1

–

–

–

–

–

–

GO:0080019

Fatty-acyl-CoA reductase (alcohol-forming) activity

–

1

–

–

–

–

–

–

GO:0015254

Glycerol channel activity

–

3

–

–

–

3

–

–

GO:0015168

Glycerol transmembrane transporter activity

–

3

–

–

–

4

–

–

GO:0090447

Glycerol-3-phosphate 2-O-acyltransferase activity

–

3

1

–

–

2

–

–

GO:0004366

Glycerol-3-phosphate O-acyltransferase activity

–

–

1

–

–

–

–

–

GO:0050062

Long-chain-fatty-acyl-CoA reductase activity

–

2

–

–

–

1

–

–

GO:0042171

Lysophosphatidic acid acyltransferase activity

–

–

–

–

–

–

1

–

GO:0071617

Lysophospholipid acyltransferase activity

–

–

–

–

–

–

1

–

GO:0008374

O-Acyltransferase activity

–

4

2

1

1

2

4

1

GO:0004607

Phosphatidylcholine-sterol O-acyltransferase activity

–

–

–

–

1

–

–

–

GO:0004623

Phospholipase A2 activity

–

–

–

–

1

–

–

–

GO:0008429

Phosphatidylethanolamine binding

–

3

–

–

–

2

–

1

GO:0004435

Phosphatidylinositol phospholipase C activity

–

1

–

–

–

–

–

–

GO:0004620

Phospholipase activity

–

1

–

–

1

1

–

1

GO:0004629

Phospholipase C activity

–

1

–

–

–

1

–

–

GO:0035091

Phosphatidylinositol binding

–

–

1

–

–

–

1

–

GO:0005543

Phospholipid binding

–

3

1

1

1

2

1

–

GO:0005548

Phospholipid transporter activity

–

–

1

–

–

–

–

–

GO:0004012

Phospholipid-translocating ATPase activity

–

–

1

–

–

–

–

–

GO:0016412

Serine O-acyltransferase activity

–

–

1

–

–

–

–

–

  1. Shown is the gene ontology (GO) annotation of the selected differentially expressed genes (DEGs) in Camelina transgenic lines overexpressing DGAT1 or GPD1 in developing seeds at 10–15 and 16–21 days after flowering (DAF). The GO ID, description and DEGs numbers are provided. Up in DG-14 or DG-21 indicate the genes up-regulated in DGAT1 #2 line in developing seeds at 10–15 and 16–21, respectively; Down in DG-14 or DG-21 indicate the genes downregulated in DGAT1 #2 line in developing seeds at 10–15 and 16–21, respectively; Up in GP-14 or GP-21 indicate the genes up-regulated in GPD1 #2 line in developing seeds at 10–15 and 16–21, respectively; Down in GP-14 or GP-21 indicate the genes downregulated in GPD1 #2 line in developing seeds at 10–15 and 16–21, respectively