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Table 1 Functional classifications of the DEGs in Camelina transgenics as compared to WT

From: Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase

GO ID GO term Counts
Up in DG-14 Down in DG-14 Up in DG-21 Down in DG-21 Up in GP-14 Down in GP-14 Up in GP-21 Down in GP-21
GO:0005488 Binding 80 236 100 152 159 198 178 211
GO:0003824 Catalytic activity 68 282 85 121 130 200 158 173
GO:1901363 Heterocyclic compound binding 24 145 57 91 93 108 116 126
GO:0016787 Hydrolase activity 21 89 30 31 42 57 61 49
GO:0016788 Hydrolase activity, acting on ester bonds 4 29 11 8 13 23 20 12
GO:0043167 Ion binding 52 149 54 87 92 119 104 84
GO:0016874 Ligase activity 4 10 2 3 2 10 1 7
GO:0008289 Lipid binding 4 8 4 1 3 8 7 4
GO:0003676 Nucleic acid binding 24 54 27 55 49 50 48 59
GO:0001071 Nucleic acid binding transcription factor activity 14 29 8 20 24 28 18 30
GO:0097159 Organic cyclic compound binding 24 145 57 91 93 108 116 126
GO:0016491 Oxidoreductase activity 16 81 15 35 31 59 31 42
GO:0005515 Protein binding 2 37 20 20 24 37 34 47
GO:0016740 Transferase activity 28 86 35 50 55 56 70 70
GO:0016746 Transferase activity, transferring acyl groups 4 15 6 6 9 9 9 12
GO:0005215 Transporter activity 12 28 14 10 18 20 22 13
GO:0016209 Antioxidant activity – 12 – 1 2 8 – –
GO:0030246 Carbohydrate binding – 6 2 6 4 5 1 6
GO:0097367 Carbohydrate derivative binding – 46 21 22 30 31 44 31
GO:0050662 Coenzyme binding – 5 4 5 2 5 7 9
GO:0048037 Cofactor binding – 7 6 6 5 7 9 11
GO:0009055 Electron carrier activity – 5 1 4 3 4 – 3
GO:0016301 Kinase activity 13 27 13 12 16 17 20 18
GO:0016829 Lyase activity 1 19 2 3 3 14 6 2
GO:0046872 Metal ion binding – 98 30 59 60 88 61 82
GO:0045735 Nutrient reservoir activity – 22 – 1 – 17 1 1
GO:0000166 Nucleotide binding – 53 26 31 36 41 58 43
GO:0019825 Oxygen binding – 14 – 2 3 8 5 3
GO:0005524 ATP binding – 44 20 19 25 27 34 28
GO:0016887 ATPase activity 7 14 4 4 7 4 9 5
GO:0008233 Peptidase activity 5 17 6 7 5 8 6 10
GO:0042578 Phosphoric ester hydrolase activity 2 9 4 4 7 12 7 5
GO:0016157 Sucrose synthase activity – 1 – – – 1 – –
GO:0046524 Sucrose-phosphate synthase activity – 1 – – – – – –
GO:0016298 Lipase activity – 6 2 – 2 3 3 1
GO:0004806 Triglyceride lipase activity – – 2 – 1 – 1 –
GO:0005319 Lipid transporter activity – 1 1 – – – – –
GO:0047617 Acyl-CoA hydrolase activity – – – – – – – 1
GO:0016411 Acylglycerol O-acyltransferase activity – 1 – 1 – – 3 –
GO:0016421 CoA carboxylase activity – – – – – –   1
GO:0047734 CDP-glycerol diphosphatase activity – – 1 – – – – –
GO:0004144 Diacylglycerol O-acyltransferase activity – 1 – 1 – – 2 –
GO:0090447 Glycerol-3-phosphate 2-O-acyltransferase activity – – – – – – 1 1
GO:0004366 Glycerol-3-phosphate O-acyltransferase activity – – – – – – 1 1
GO:0052722 Fatty acid in-chain hydroxylase activity – 1 – – – – – –
GO:0080019 Fatty-acyl-CoA reductase (alcohol-forming) activity – 1 – – – – – –
GO:0015254 Glycerol channel activity – 3 – – – 3 – –
GO:0015168 Glycerol transmembrane transporter activity – 3 – – – 4 – –
GO:0090447 Glycerol-3-phosphate 2-O-acyltransferase activity – 3 1 – – 2 – –
GO:0004366 Glycerol-3-phosphate O-acyltransferase activity – – 1 – – – – –
GO:0050062 Long-chain-fatty-acyl-CoA reductase activity – 2 – – – 1 – –
GO:0042171 Lysophosphatidic acid acyltransferase activity – – – – – – 1 –
GO:0071617 Lysophospholipid acyltransferase activity – – – – – – 1 –
GO:0008374 O-Acyltransferase activity – 4 2 1 1 2 4 1
GO:0004607 Phosphatidylcholine-sterol O-acyltransferase activity – – – – 1 – – –
GO:0004623 Phospholipase A2 activity – – – – 1 – – –
GO:0008429 Phosphatidylethanolamine binding – 3 – – – 2 – 1
GO:0004435 Phosphatidylinositol phospholipase C activity – 1 – – – – – –
GO:0004620 Phospholipase activity – 1 – – 1 1 – 1
GO:0004629 Phospholipase C activity – 1 – – – 1 – –
GO:0035091 Phosphatidylinositol binding – – 1 – – – 1 –
GO:0005543 Phospholipid binding – 3 1 1 1 2 1 –
GO:0005548 Phospholipid transporter activity – – 1 – – – – –
GO:0004012 Phospholipid-translocating ATPase activity – – 1 – – – – –
GO:0016412 Serine O-acyltransferase activity – – 1 – – – – –
  1. Shown is the gene ontology (GO) annotation of the selected differentially expressed genes (DEGs) in Camelina transgenic lines overexpressing DGAT1 or GPD1 in developing seeds at 10–15 and 16–21 days after flowering (DAF). The GO ID, description and DEGs numbers are provided. Up in DG-14 or DG-21 indicate the genes up-regulated in DGAT1 #2 line in developing seeds at 10–15 and 16–21, respectively; Down in DG-14 or DG-21 indicate the genes downregulated in DGAT1 #2 line in developing seeds at 10–15 and 16–21, respectively; Up in GP-14 or GP-21 indicate the genes up-regulated in GPD1 #2 line in developing seeds at 10–15 and 16–21, respectively; Down in GP-14 or GP-21 indicate the genes downregulated in GPD1 #2 line in developing seeds at 10–15 and 16–21, respectively
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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