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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

Fig. 1

Effect of energy delivered via poring and transfer pulses on transformation efficiency of strain KJ. Energies delivered via poring pulse and transfer pulses were recorded for each electroporation. The number of Zeocin™-resistant clones obtained after electroporation of 3.0 × 107 cells with 1, 2 or 4 µg DNA of the pble-PeEGFP-KE1E plasmid was counted in each experiment; the transformation efficiency was calculated by dividing the number of Zeocin™-resistant clones by the number of input cells (3.0 × 107). A total of 97 electroporation experiments were performed; all 97 results (Additional file 1) are incorporated in each of the panels (a) and (b). a The relationship between the transformation efficiency and energy delivered via the poring pulse. b The relationship between the transformation efficiency and energy delivered via the transfer pulse

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