Fig. 3

Localization of fluorescently labeled Cas9 proteins in strain KJ cells by confocal laser scanning microscopy. Fluorescently labeled Cas9 proteins were mixed with strain KJ cells in an electroporation cuvette; the cells were electroporated or not electroporated. Subsequently, the cells were washed five times with PBS buffer, and cells containing HiLyte Fluor™ 555-Cas9 were examined microscopically. Micrographs of two electroporated (a, b) and two non-electroporated (c, d) cells are shown. The micrographs represent pseudo-colored images: fluorescence of chlorophyll, SYTO 9, and labeled Cas9 proteins are shown in blue, green, and red, respectively. The scale bar indicates 1 μm