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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

Fig. 5

Phenotypic analysis of KJFTSY knockout lines. The wild-type strain and four knockout strains were cultured in MA5 liquid medium under continuous light (100 μmol photons m−2 s−1) with 1% (v/v) CO2 bubbling. The photograph of 7-day cultures were taken after adjusting their densities to OD720 = 5.0 (a). Chlorophyll content per algal dry weight (w/w) (b) and a/b ratio (c) were quantified at day 4 while growth curves (d) were followed for 7 days. Each experiment was conducted in biological triplicate, and the mean ± SE were calculated. The asterisks on the top of the histograms indicate a statistically significant difference of P < 0.01 using Student’s t test

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