Table 2 Differentially expressed genes involved in central carbon metabolism and lipid metabolism in T. minus under photoautotrophic and heterotrophic conditions
Pathway | Gene symbol | Gene name | EC number | Fold change |
|---|---|---|---|---|
Carbon metabolism | GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | 1.2.1.12 | − 1.08 |
PFK | 6-Phosphofructokinase 1 | 2.7.1.11 | − 3.22 | |
PK | Pyruvate kinase | 2.7.1.40 | − 1.25 | |
PDH | Pyruvate dehydrogenase E1 component alpha subunit | 1.2.4.1 | − 1.84 | |
FBP | Fructose-1,6-bisphosphatase | 3.1.3.11 | + 3.82 | |
ENO | Enolase | 4.2.1.11 | − 1.75 | |
PGK | Phosphoglycerate kinase | 2.7.2.3 | − 1.67 | |
DLD | Dihydrolipoamide dehydrogenase | 1.8.1.4 | − 1.10 | |
PEPC | Phosphoenolpyruvate carboxylase | 4.1.1.39 | + 2.89 | |
ACOS | Acetyl-CoA synthetase | 6.2.1.1 | − 1.53 | |
GDH | Glutamate dehydrogenase (NAD(P)+) | 1.4.1.2 | + 3.27 | |
MDH | Malic dehydrogenase | 1.1.1.37 | + 1.66 | |
TKT | Transketolase | 2.2.1.1 | − 1.65 | |
PGDH | Phosphogluconate dehydrogenase | 1.1.1.343 | − 1.41 | |
Fatty acid biosynthesis | ACC | Acetyl-CoA carboxylase | 6.4.1.2 | − 1.24 |
KASIII | 3-Ketoacyl-CoA sythase III | 2.3.1.180 | − 1.14 | |
LACS | Long-chain acyl-CoA synthetase | 6.2.1 | − 1.29 | |
SAD | Stearoyl-ACP reductase | 1.14.19.2 | + 1.24 | |
FAD | Acyl-lipid omega-6 desaturase | 1.14.19 | − 1.48 | |
Triacylglycerol biosynthesis | GPAT | Glycerol-3-phosphate acyltransferase | 2.3.1.15 | − 0.35 |
DGK | Diacylglycerol kinase | 2.7.1.107 | − 1.46 |