Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment

Cheng, Pengfei; Zhou, Chengxu; Wang, Yan; Xu, Zhihui; Xu, Jilin; Zhou, Dongqing; Zhang, Yinghui; Wu, Haizhen; Zhang, Xuezhi; Liu, Tianzhong; Tang, Ming; Yang, Qiyong; Yan, Xiaojun; Fan, Jianhua

doi:10.1186/s13068-018-1331-5

Biotechnology for Biofuels and Bioproducts

Table 2 List of annotated enzymes that potentially involved in fatty acid-derived hydrocarbon biosynthesis and TAG assembly

From: Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment

EC #	Enzyme description	Unigene number	LogFC (2 days)^a	LogFC (4 days)	LogFC (8 days)
Fatty acid biosynthesis
6.4.1.2	Acetyl-CoA carboxylase carboxyl transferase	17	0.16	− 0.51	0.12
6.3.4.14	Acetyl-CoA carboxylase/biotin carboxylase	27	− 0.17	− 0.61	0.04
2.3.1.39	[Acyl-carrier-protein] S-malonyltransferase	8	0.24	− 0.26	0.30
1.1.1.100	3-Oxoacyl-[acyl-carrier protein] reductase	52	0.01	− 0.21	− 0.25
2.3.1.179	3-Oxoacyl-[acyl-carrier-protein] synthase II	24	0.37	− 0.07	− 0.13
2.3.1.180	3-Oxoacyl-[acyl-carrier-protein] synthase III	6	0.83	0.31	− 0.58
1.14.19.2	Acyl-[acyl-carrier-protein] desaturase	6	0.40	0.21	− 0.03
1.14.19.1	Stearoyl-CoA desaturase (delta-9 desaturase)	23	0.95	0.39	− 0.69
3.1.2.14	Fatty acyl-ACP thioesterase A/oleoyl-ACP hydrolase	4	0.80	0.01	− 0.25
2.3.1.85	Fatty acid synthase, animal type	17	0.33	0.60	− 0.70
6.2.1.3	Long-chain acyl-CoA synthetase	95	0.14	− 0.22	− 0.10
6.2.1.3	Long-chain fatty acid-CoA ligase	10	1.03	0.98	− 0.74
3.1.2.21	Medium-chain acyl-[acyl-carrier-protein] hydrolase	1	− 0.15	− 1.02	1.09
Fatty acid desaturation and elongation
1.14.19.6	Omega-6 fatty acid desaturase (delta-12 desaturase)	15	0.13	− 0.70	− 0.93
1.14.19.45	Omega-6 fatty acid desaturase (delta-12 desaturase)	16	0.81	0.09	0.09
2.3.1.199	3-Ketoacyl-CoA synthase	50	0.48	0.18	− 0.45
1.1.1.35	3-Hydroxyacyl-CoA dehydrogenase	5	4.20	1.52	− 3.09
1.1.1.211	Enoyl-CoA hydratase/long-chain 3-hydroxyacyl-CoA dehydrogenase	17	2.53	1.44	− 1.23
2.3.1.199	Fatty acid elongase	37	1.11	0.02	− 1.32
4.2.1.134	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	16	0.56	0.62	− 0.73
1.3.1.38	Trans-2-enoyl-CoA reductase	22	0.73	0.36	− 0.75
1.3.1.93	Very-long-chain enoyl-CoA reductase	9	0.75	0.37	− 0.55
Fatty acid oxidation
4.1.1.9	Malonyl-CoA decarboxylase	7	− 0.04	0.06	− 0.44
2.3.1.16	Acetyl-CoA acyltransferase	33	0.69	0.82	− 0.67
3.1.2.2	Acyl-coenzyme A thioesterase	1	N.D.^b	N.D.	− 3.05
1.3.3.6	Acyl-CoA oxidase	53	0.22	0.05	− 0.10
1.3.99.12	Short/branched chain acyl-CoA dehydrogenase	7	0.26	0.16	− 2.4
1.3.8.9	Very long chain acyl-CoA dehydrogenase	5	0.57	0.26	− 1.02
1.3.8.7	Acyl-CoA dehydrogenase	25	0.08	− 0.14	− 2.43
4.2.1.17	Enoyl-CoA hydratase	26	− 0.04	− 0.49	− 0.55
2.3.1.9	Acetyl-CoA C-acetyltransferase	28	0.46	0.40	− 1.28
4.2.1.119	3-Hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase 2	15	− 0.08	0.06	0.00
TAG synthesis
2.7.1.30	Glycerol kinase	12	0.39	0.02	− 0.20
2.3.1.15	Glycerol-3-phosphate O-acyltransferase	17	0.04	− 0.30	− 0.27
2.3.1.51	Lysophospholipid acyltransferase	17	0.68	− 0.22	− 1.51
3.1.3.4	Diacylglycerol diphosphate phosphatase/phosphatidate phosphatase	33	− 0.31	− 0.33	− 0.15
2.3.1.20	Diacylglycerol O-acyltransferase/wax-ester synthase	1	N.D.	2.32	− 1.97
2.3.1.158	Phospholipid:diacylglycerol acyltransferase	9	0.52	0.72	− 0.31

^a Summation of all FPKM values of unigenes that associated with the same enzyme were used for logFC calculation
^b N.D. means could not detect
^c More than twofold increase or decrease were marked as italic type

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