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Table 2 List of annotated enzymes that potentially involved in fatty acid-derived hydrocarbon biosynthesis and TAG assembly

From: Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment

EC # Enzyme description Unigene number LogFC (2 days)a LogFC (4 days) LogFC (8 days)
Fatty acid biosynthesis
 6.4.1.2 Acetyl-CoA carboxylase carboxyl transferase 17 0.16 − 0.51 0.12
 6.3.4.14 Acetyl-CoA carboxylase/biotin carboxylase 27 − 0.17 − 0.61 0.04
 2.3.1.39 [Acyl-carrier-protein] S-malonyltransferase 8 0.24 − 0.26 0.30
 1.1.1.100 3-Oxoacyl-[acyl-carrier protein] reductase 52 0.01 − 0.21 − 0.25
 2.3.1.179 3-Oxoacyl-[acyl-carrier-protein] synthase II 24 0.37 − 0.07 − 0.13
 2.3.1.180 3-Oxoacyl-[acyl-carrier-protein] synthase III 6 0.83 0.31 − 0.58
 1.14.19.2 Acyl-[acyl-carrier-protein] desaturase 6 0.40 0.21 − 0.03
 1.14.19.1 Stearoyl-CoA desaturase (delta-9 desaturase) 23 0.95 0.39 − 0.69
 3.1.2.14 Fatty acyl-ACP thioesterase A/oleoyl-ACP hydrolase 4 0.80 0.01 − 0.25
 2.3.1.85 Fatty acid synthase, animal type 17 0.33 0.60 − 0.70
 6.2.1.3 Long-chain acyl-CoA synthetase 95 0.14 − 0.22 − 0.10
 6.2.1.3 Long-chain fatty acid-CoA ligase 10 1.03 0.98 − 0.74
 3.1.2.21 Medium-chain acyl-[acyl-carrier-protein] hydrolase 1 − 0.15 − 1.02 1.09
Fatty acid desaturation and elongation
 1.14.19.6 Omega-6 fatty acid desaturase (delta-12 desaturase) 15 0.13 − 0.70 − 0.93
 1.14.19.45 Omega-6 fatty acid desaturase (delta-12 desaturase) 16 0.81 0.09 0.09
 2.3.1.199 3-Ketoacyl-CoA synthase 50 0.48 0.18 − 0.45
 1.1.1.35 3-Hydroxyacyl-CoA dehydrogenase 5 4.20 1.52 − 3.09
 1.1.1.211 Enoyl-CoA hydratase/long-chain 3-hydroxyacyl-CoA dehydrogenase 17 2.53 1.44 − 1.23
 2.3.1.199 Fatty acid elongase 37 1.11 0.02 − 1.32
 4.2.1.134 Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 16 0.56 0.62 − 0.73
 1.3.1.38 Trans-2-enoyl-CoA reductase 22 0.73 0.36 − 0.75
 1.3.1.93 Very-long-chain enoyl-CoA reductase 9 0.75 0.37 − 0.55
Fatty acid oxidation
 4.1.1.9 Malonyl-CoA decarboxylase 7 − 0.04 0.06 − 0.44
 2.3.1.16 Acetyl-CoA acyltransferase 33 0.69 0.82 − 0.67
 3.1.2.2 Acyl-coenzyme A thioesterase 1 N.D.b N.D. − 3.05
 1.3.3.6 Acyl-CoA oxidase 53 0.22 0.05 − 0.10
 1.3.99.12 Short/branched chain acyl-CoA dehydrogenase 7 0.26 0.16 − 2.4
 1.3.8.9 Very long chain acyl-CoA dehydrogenase 5 0.57 0.26 − 1.02
 1.3.8.7 Acyl-CoA dehydrogenase 25 0.08 − 0.14 − 2.43
 4.2.1.17 Enoyl-CoA hydratase 26 − 0.04 − 0.49 − 0.55
 2.3.1.9 Acetyl-CoA C-acetyltransferase 28 0.46 0.40 − 1.28
 4.2.1.119 3-Hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase 2 15 − 0.08 0.06 0.00
TAG synthesis
 2.7.1.30 Glycerol kinase 12 0.39 0.02 − 0.20
 2.3.1.15 Glycerol-3-phosphate O-acyltransferase 17 0.04 − 0.30 − 0.27
 2.3.1.51 Lysophospholipid acyltransferase 17 0.68 − 0.22 − 1.51
 3.1.3.4 Diacylglycerol diphosphate phosphatase/phosphatidate phosphatase 33 − 0.31 − 0.33 − 0.15
 2.3.1.20 Diacylglycerol O-acyltransferase/wax-ester synthase 1 N.D. 2.32 − 1.97
 2.3.1.158 Phospholipid:diacylglycerol acyltransferase 9 0.52 0.72 − 0.31
  1. a Summation of all FPKM values of unigenes that associated with the same enzyme were used for logFC calculation
  2. b N.D. means could not detect
  3. c More than twofold increase or decrease were marked as italic type
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Biotechnology for Biofuels

ISSN: 1754-6834

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