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Table 3 Specific activity of EG1 and its variants on different substrates with added lignin

From: Altering the linker in processive GH5 endoglucanase 1 modulates lignin binding and catalytic properties

Enzymes

Specific activity (U/μmol)

MMPL lignin

MPKL lignin

CMC-Na

RAC

FP

CMC-Na

RAC

FP

EG1

1261.30 ± 16.89

495.17 ± 6.19

7.26 ± 0.15

1292.53 ± 27.05

316.61 ± 0.52

6.65 ± 0.65

EG1-Δ10

918.91 ± 18.36

345.24 ± 9.91

4.62 ± 0.17

978.45 ± 39.16

234.99 ± 0.61

4.41 ± 0.33

EG1-Δ19

210.78 ± 10.63

135.68 ± 3.53

4.04 ± 0.15

190.43 ± 23.57

128.18 ± 8.91

3.67 ± 0.51

EG1-A(EAAAK)2A

1327.70 ± 27.81

399.80 ± 1.25

7.06 ± 0.05

1267.59 ± 38.58

353.40 ± 9.23

6.77 ± 0.33

EG1CD

1311.03 ± 21.48

437.39 ± 4.17

6.80 ± 0.09

1170.26 ± 42.84

338.60 ± 5.10

6.54 ± 0.46

EG1-ApCel5A

1444.70 ± 44.14

506.02 ± 5.51

7.06 ± 0.05

1463.72 ± 41.71

464.94 ± 4.29

6.44 ± 0.24

EG1-L1

1243.79 ± 32.34

427.88 ± 5.34

7.05 ± 0.06

1267.50 ± 20.34

427.4 ± 10.72

6.51 ± 0.48

EG1-(P→G)

1110.46 ± 19.56

301.56 ± 18.90

6.11 ± 0.52

1044.81 ± 27.98

282.03 ± 1.23

6.11 ± 0.77

EG1-(G→P)

1356.56 ± 31.45

472.45 ± 31.88

8.10 ± 0.77

1273.92 ± 33.19

419.82 ± 2.11

8.02 ± 0.15

  1. Values shown are means of triplicate determinations ± standard error (SE)