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Table 3 Specific activity of EG1 and its variants on different substrates with added lignin

From: Altering the linker in processive GH5 endoglucanase 1 modulates lignin binding and catalytic properties

Enzymes Specific activity (U/μmol)
MMPL lignin MPKL lignin
CMC-Na RAC FP CMC-Na RAC FP
EG1 1261.30 ± 16.89 495.17 ± 6.19 7.26 ± 0.15 1292.53 ± 27.05 316.61 ± 0.52 6.65 ± 0.65
EG1-Δ10 918.91 ± 18.36 345.24 ± 9.91 4.62 ± 0.17 978.45 ± 39.16 234.99 ± 0.61 4.41 ± 0.33
EG1-Δ19 210.78 ± 10.63 135.68 ± 3.53 4.04 ± 0.15 190.43 ± 23.57 128.18 ± 8.91 3.67 ± 0.51
EG1-A(EAAAK)2A 1327.70 ± 27.81 399.80 ± 1.25 7.06 ± 0.05 1267.59 ± 38.58 353.40 ± 9.23 6.77 ± 0.33
EG1CD 1311.03 ± 21.48 437.39 ± 4.17 6.80 ± 0.09 1170.26 ± 42.84 338.60 ± 5.10 6.54 ± 0.46
EG1-ApCel5A 1444.70 ± 44.14 506.02 ± 5.51 7.06 ± 0.05 1463.72 ± 41.71 464.94 ± 4.29 6.44 ± 0.24
EG1-L1 1243.79 ± 32.34 427.88 ± 5.34 7.05 ± 0.06 1267.50 ± 20.34 427.4 ± 10.72 6.51 ± 0.48
EG1-(P→G) 1110.46 ± 19.56 301.56 ± 18.90 6.11 ± 0.52 1044.81 ± 27.98 282.03 ± 1.23 6.11 ± 0.77
EG1-(G→P) 1356.56 ± 31.45 472.45 ± 31.88 8.10 ± 0.77 1273.92 ± 33.19 419.82 ± 2.11 8.02 ± 0.15
  1. Values shown are means of triplicate determinations ± standard error (SE)