Fig. 3

Mycolic acid composition of R. opacus strains using glucose or phenol as a sole carbon source. a Heat map of double bond (DB) numbers for mycolic acids (MAs) in the WT and evol40 strains. The values shown in the heat map are the average of three replicates. See color bar for scale. The DB number represents the total number of double bonds and cyclopropane units on acyl chains. b Heat map of MA carbon (C) number distribution, defined as the total number of carbons located on acyl chains. The values shown in the heat map are the average of three replicates. See color bar for scale. WTG WT strain grown in 1 g/L glucose (glucose), 40G evol40 grown in glucose, WTLP WT strain grown in 0.75 g/L phenol (low phenol), 40LP evol40 strain grown in low phenol, 40HP evol40 strain grown in 1.5 g/L phenol (high phenol). MA percentage is defined as the total ion counts of each category (DB number or C number) divided by the total ion counts of all mycolic acids detected in each sample.