Phospholipid composition of R. opacus strains using glucose or phenol as a sole carbon source. a Ratio of phosphatidylinositol (PI) to phosphatidylethanolamine (PE) total ion counts in the WT and evol40 strains using glucose or phenol as a sole carbon source. b Percentage of unsaturated phospholipids (PLs) (i.e., phospholipids with at least one unsaturated fatty acyl substituent) in WT and evol40 strains using glucose or phenol as a sole carbon source. c Distribution of phospholipid carbon (C) numbers in WT and evol40 strains. Here, C number is defined as the total number of acyl chain carbons, and unsaturation means double bonds and cyclic chains on acyl substituents. Each square is the average of three replicates. PL percentage is defined as the total ion counts of each category (unsaturated phospholipids or C number) divided by the total ion counts of all detected PI and PE species in the sample. For A and B, bars represent the average of three replicates, error bars represent one standard deviation, and statistical significance was determined using a one mean, two-tailed Student’s t test (*P < 0.05; **P < 0.01). WTG WT strain grown in 1 g/L glucose (glucose), 40G evol40 grown in glucose, WTLP WT strain grown in 0.75 g/L phenol (low phenol), 40LP evol40 strain grown in low phenol, 40HP evol40 strain grown in 1.5 g/L phenol (high phenol).