Fig. 8

Expression profiles of TAG biosynthesis genes in PLDα1KD and wild-type developing seeds. a Kennedy pathway for TAG biosynthesis in the ER. b Expression of major genes involved in the Kennedy pathway in soybean developing seeds of different genetic backgrounds under various growth conditions. The X-axis numbers indicated each development stage. KD1-h, KD2-h and WT-h: PLDα1 knockdown line 1, 2 and wild-type Jack, respectively, under high temperature and humidity which used thick lines; KD1-n, KD2-n and WT-n: PLDα1 knockdown line 1, 2 and wild-type Jack, respectively, under normal temperature and humidity which used thin lines. 1, 2, 3, 4, and 5 indicate different developing stages of seeds, corresponding to fresh weights as described previously. The values are the mean ± SD (n = 3). * and **Denote significance at P < 0.05 and P < 0.01, respectively, compared with wild-type Jack (WT) based on Student’s t test