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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Discovery of novel geranylgeranyl reductases and characterization of their substrate promiscuity

Fig. 3

(Top) normalized TIC profiles of five putative GGRs (Af, red; Pf, green; Mi, blue; Sa, magenta; Tn, orange; no enzyme, black) found to reduce GGOH (RT = 8.31 min) to H2-GGOH (RT = 8.12 min), H4-GGOH (RT = 7.85 min), H6-GGOH (RT = 7.72 min), and H8-GGOH (RT = 7.52 min) upon 1 h incubation under standard assay conditions. All peaks elute with a relative error of ± 0.05 min. (Bottom) the associated mass spectra for GGOH (blue), H2-GGOH (green), H4-GGOH (red), H6-GGOH (orange), H8-GGOH (gray) are shown with signature ions used for structural assignment of products

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