Fig. 4
a Cumulative 1D 1H NMR spectra (n = 6) of Scenedesmus sp. IITRIND2 Control polar extracts (pink) stacked up with that of ASW cultures (blue). The spectral peaks were assigned for particular small-molecule metabolites. The water region at δ 4.6–4.9 was removed for clarity. The abbreviations used are: 3-OH-IV: 3-hydroxyisovalerate; Gln: glutamine; Glu: glutamate; EA: ethanolamine; Cys: cysteine; Cho: choline; PC: phosphocholine; GPC: glycerophosphocholine; DSS: 4,4-dimethyl-4-silapentane-1-sulfonic acid; TMAO: trimethylamine-N-oxide; DMG: N,N-dimethylglycine; Phe: phenylalanine; ATP: adenosine triphosphate; UDP glucose: uridine diphosphate glucose. b Combined PCA 2D score plot resulted from the analysis of 1D 1H CPMG spectra of Scenedesmus sp. IITRIND2 cultivated in control and ASW medium (green = control; red = salt). The semi-transparent red and green ovals represent the 95% confidence interval. c PCA loadings plot revealing the metabolites of responsible for the discrimination pattern, the more the metabolite is away from the origin (0,0) more it contributes in the group discrimination