Fig. 3From: Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803Confirmation of each gene location by PCR analysis using specific pairs of primers (Table 2) in each engineered strain including OXAas (A), OXAas/KOAar (B) and OXAccDACB/KOLipA (C) strains in this study. The location of aas gene fragment in OXAas was checked using a pair of pE_SF and pE_SR primers for pEERM core structure (a). Lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–10: clone numbers 1 to 9, For Cm_SF and Aas_SR (b) primer, lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–6: clone numbers 1 to 5. In (c), the pair of Aas_F6 and Cm_SR (c) primers was used, lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–6: clone numbers 1 to 5. The UUPSF_Aas and Cm_SR (d) primer, lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–6: clone numbers 1 to 5. Confirmation of gene location in OXAas/KOAar (B) using a pair of CAar_F and Aas_SR (a) primer, Lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–9; clone numbers 1 to 8 whereas UUPSF_Aar and Aas_SR (b) primers was used. Lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–11: clone numbers 1 to 10. The gene location in OXAccDACB/KOLipA (C) using pair of UUPSF_lipA and AccD_SR primer, Lane M: GeneRuler™ DNA ladder (Fermentas) and lanes 1–5; clone numbers 1 to 5Back to article page