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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803

Fig. 3

Confirmation of each gene location by PCR analysis using specific pairs of primers (Table 2) in each engineered strain including OXAas (A), OXAas/KOAar (B) and OXAccDACB/KOLipA (C) strains in this study. The location of aas gene fragment in OXAas was checked using a pair of pE_SF and pE_SR primers for pEERM core structure (a). Lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–10: clone numbers 1 to 9, For Cm_SF and Aas_SR (b) primer, lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–6: clone numbers 1 to 5. In (c), the pair of Aas_F6 and Cm_SR (c) primers was used, lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–6: clone numbers 1 to 5. The UUPSF_Aas and Cm_SR (d) primer, lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–6: clone numbers 1 to 5. Confirmation of gene location in OXAas/KOAar (B) using a pair of CAar_F and Aas_SR (a) primer, Lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–9; clone numbers 1 to 8 whereas UUPSF_Aar and Aas_SR (b) primers was used. Lane M: GeneRuler™ DNA ladder (Fermentas), lane 1: negative control using WT as template and lanes 2–11: clone numbers 1 to 10. The gene location in OXAccDACB/KOLipA (C) using pair of UUPSF_lipA and AccD_SR primer, Lane M: GeneRuler™ DNA ladder (Fermentas) and lanes 1–5; clone numbers 1 to 5

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