Skip to main content

Table 1 Strains and plasmids used in this study

From: Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803

Name Relevant genotype Reference
Cyanobacterial strains
 Synechocystis sp. PCC 6803 Wild type Pasteur culture collection
 OXAas aas, cmr integrated at region of native aas gene in Synechocystis genome This study
 OXAccDACB/KOLipA accDACB, cmr integrated at flanking region of lipA gene in Synechocystis genome This study
 OXAas/KOAar aas, cmr integrated at flanking region of aar gene in Synechocystis genome This study
Plasmids
 pEERM PpsbA2-cmr; plasmid containing flanking region of psbA2 gene Englund et al. [18]
 pEERM_Aas PpsbA2-aas-cmr; integrated between XbaI and SpeI sites of pEERM This study
 pEERM_LipA/AccDACB PpsbA2-USlipA-accDACB-DWlipA-cmr; integrated between XbaI and SpeI sites of pEERM This study
 pEERM_Aar/Aas PpsbA2-USaar-aas-DWaar-cmr; integrated between XbaI and SpeI sites of pEERM This study
  1. PpsbA2, strong psbA2 promoter; cmr, chloramphenicol resistance cassette