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Table 1 Strains and plasmids used in this study

From: Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803

Name

Relevant genotype

Reference

Cyanobacterial strains

 Synechocystis sp. PCC 6803

Wild type

Pasteur culture collection

 OXAas

aas, cmr integrated at region of native aas gene in Synechocystis genome

This study

 OXAccDACB/KOLipA

accDACB, cmr integrated at flanking region of lipA gene in Synechocystis genome

This study

 OXAas/KOAar

aas, cmr integrated at flanking region of aar gene in Synechocystis genome

This study

Plasmids

 pEERM

PpsbA2-cmr; plasmid containing flanking region of psbA2 gene

Englund et al. [18]

 pEERM_Aas

PpsbA2-aas-cmr; integrated between XbaI and SpeI sites of pEERM

This study

 pEERM_LipA/AccDACB

PpsbA2-USlipA-accDACB-DWlipA-cmr; integrated between XbaI and SpeI sites of pEERM

This study

 pEERM_Aar/Aas

PpsbA2-USaar-aas-DWaar-cmr; integrated between XbaI and SpeI sites of pEERM

This study

  1. PpsbA2, strong psbA2 promoter; cmr, chloramphenicol resistance cassette