Skip to main content

Table 2 Primers used for PCR amplification, sequencing and determination of gene location

From: Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803

Name

Sequences

UPAar_F

5′-AGATCTAGGGACGGAACAAACCCTCCAAAGC-3′

UPAar_R1

5′-GAAGATCCTTTGATTTTGCCGACAGGATAGGGCGTGTGTGGA-3′

UPAar_R2

5′-GAATTCAAAAAAAGGATCTCAAGAAGATCCTTTGATTTTGCCGACAGGA-3′

DWAar_F

5′-GGATCCCATTGATAATAGTCAGAATAAATAG-3′

DWAar_R

5′-GTCGACCCTTTAGTAGCTCTTTAGGGGTTAA-3′

UPlipA_F

5′-TAGAGAAGATCT CAGGCCCTACGTCGTCATAATCCTG -3′

UPlipA_R1

5′-GAAGATCCTTTGATTTTGTGGATTGGAAAGGGATTAGTCTTC-3′

UPlipA_R2

5′-TAGAGAGAATTCAAAAAAAGGATCTCAAGAAGATCCTTTGATTTTGTGGATTGGA-3′

DWlipA_F

5′-TAGAGAGGATCCTAGGTTCTACAAACTCAGCAAACGG-3′

DWlipA_R

5′-TAGAGAGTCGACAGGTCAACCAAGATTCGGTGCACCA-3′

AccA_F

5′-TCTAGATAGTGGAGGTACTAGAATGAGTAAAAGTGAGCGTCGTG-3′

AccA_R

5′-CTGCAGCGGCCGCTACTAGTTTACACCGCCGTTTCTAAAAATTG-3′

AccB_F

5′-TCTAGATAGTGGAGGTACTAGAATGGACTACAAGGATGACGATGACAAG-3′

AccB_R

5′-CTGCAGCGGCCGCTACTAGTCTAGGGTTTAATCCACATTAGGG-3′

AccC_F

5′-TCTAGATAGTGGAGGTACTAGAATGCAATTCGCCAAAATTTTAATTGCC-3′

AccC_R

5′-CTGCAGCGGCCGCTACTAGTCTAGGGTGTTAAATGCTCTTCG-3′

AccD_F

5′-TCTAGATAGTGGAGGTACTAGAATGTCTCTATTTGATTGGTTTG-3′

AccD_R

5′-CTGCAGCGGCCGCTACTAGTTTAACCATCTTGATTGACGGAAA-3′

UUPPsbA2_SF

5′-GTGATGCCTGTCAGCAAAACAACTT-3′

Aas_F3

5′-AGACAATCTAGAGTGGACAGTGGCCAT-3′

Aas_R5

5′-GGAGATGGTTCAAGCTCAGG-3′

Aas_F4

5′-ACTCCCTAGAAAGAAGCGCC-3′

Aas_R6

5′-ATAAACACTAGTTTAAAACATTTCGTC-3′

Aas_SR

5′-GGCTATTCCAATGGATTTGAGGTTG-3′

Cm_SF

5′-GGCAGAATGCTTAATGAATTACAACAG-3′

Cm_SR

5′-CTGAAATGCCTCAAAATGTTCTTTACG-3′

UUPF_Aas

5′-GCGATCGCCGTCAATTTTCGATCAG-3′

pE_SF

5′-CATTACGCTGACTTGACGGG-3′

pE_SR

5′-AGGTATGTAGGCGGTGCTAC-3′

UUPF_LipA

5′-ACAGGGCCAGGTGGGAGAAATTTTG-3′

AccA_SR

5′-CTACCGGCCAATCAAGTTTGCAC-3′

UUPF_Aar

5′-CAAAAGTAATGAGGTCGTTTTACCC-3′

CUPAar_SF

5′-CTACCGGCCAATCAAGTTTGCAC-3′