Table 3 Primer used for RT-PCR reactions
Target gene | Name | Primers | PCR product size (bp) |
|---|---|---|---|
16s | 16s_F | 5′-AGTTCTGACGGTACCTGATGA-3′ | 521 |
16s_R | 5′-GTCAAGCCTTGGTAAGGTTAT-3′ | ||
plsX | PlsX_F | 5′-AAGGGGTGGTGGAAATGGAA-3′ | 488 |
PlsX _R | 5′-AAGTAGGTCCCTTCCTTCGG-3′ | ||
accA | AccA_F | 5′-ATGCACGGCGATCGAGGAGGT-3′ | 428 |
AccA_R | 5′-TGGAGTAGCCACGGTGTACAC-3′ | ||
aas | Aas_F_RT | 5′-CCCATTGAAGATGCCTGTTT-3′ | 304 |
Aas_R _RT | 5′-GTGCTGGGATAAAACGGAAA-3′ | ||
phaA | PhaA_F | 5′-TCAGCCGGATAGAATTGGACGAAGT-3′ | 432 |
PhaA_R | 5′-CAAACAAGTCAAAATCTGCCAGGGTT-3′ | ||
lipA | LipA_F | 5′-TTGGCGGAGCAAGTGAAGCAAT-3′ | 379 |
LipA_R | 5′-CATGGACCAGCACAGGCAAAAT-3′ | ||
aar | Aar_F | 5′-GGGAGATATTGGTAGCGCCG-3′ | 394 |
Aar_R | 5′-CCGCAAAACAGGCGAACATT-3′ |