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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Overproduction of native endo-β-1,4-glucanases leads to largely enhanced biomass saccharification and bioethanol production by specific modification of cellulose features in transgenic rice

Fig. 2

Transformation of OsGH9B1 and OsGH9B3 produced overexpressed proteins in the transgenic lines. a Constructs used for overexpression of the OsGH9B1 and OsGH9B3 genes. b Gene expression in WT (Nipponbare) plants and four transgenic lines. c Detection of OsGH9B1 and OsGH9B3 proteins in total proteins samples extracted from stem tissues. d The distribution of proteins in soluble protein samples (Sup), plasma membrane protein (PMy, and total protein samples (Res). e Observation of OsGH9B1/B3-eGFP in protoplast, scale bar as 10 μm. f Cellulase activity assay in vitro using total proteins extracted from stem tissues; Student’s t test performed for WT plants and transgenic lines as **p < 0.01 and *p < 0.05

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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