Fig. 1

Multiple amino acid sequence alignment of FsAlgB and other alginate lyases of PL 7 family: AlyA (AAA25049) from Klebsiella pneumoniae subsp. Aerogenes, AlyA5 (CAZ96266) from Zobellia galactanivorans DsiJT, PA1167 (AAG04556) from Pseudomonas aeruginosa PAO1, AlyPG (BAA83339) from Corynebacterium sp. ALY-1, AlyA1 (CAZ95239) from Zobellia galactanivorans DsiJT, and A1-II′ (BAD16656) from Sphingomonas sp. A1. Identical and similar amino acid residues among the alginate lyases are shaded in yellow. The locations of three conserved regions are marked and the key amino acid “I” is marked with red star. SA3-5 indicated the common strands located in the sequence alignments