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Table 1 Substrate specificity and kinetics of FsAlgB (data shown are the mean ± SD, n = 3)

From: Elucidation of degrading pattern and substrate recognition of a novel bifunctional alginate lyase from Flammeovirga sp. NJ-04 and its use for preparation alginate oligosaccharides

Substrate Sodium alginate polyMG polyM polyG
Activity (U/mg) 1760.8 ± 15.8 2103.2 ± 21.3 1124.9 ± 8.2 2445.6 ± 12.7
Km (mM) 1.28 ± 0.28 0.92 ± 0.04 2.06 ± 0.17 0.69 ± 0.11
Vmax (nmol/s) 0.12 ± 0.03 0.15 ± 0.01 0.08 ± 0.01 0.18 ± 0.04
kcat (s−1) 2.22 ± 0.19 2.73 ± 0.23 1.48 ± 0.07 3.33 ± 0.21
kcat/Km (s−1/mM) 1.73 ± 0.32 2.97 ± 0.41 0.72 ± 0.17 4.83 ± 0.39