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Table 1 Substrate specificity and kinetics of FsAlgB (data shown are the mean ± SD, n = 3)

From: Elucidation of degrading pattern and substrate recognition of a novel bifunctional alginate lyase from Flammeovirga sp. NJ-04 and its use for preparation alginate oligosaccharides

Substrate

Sodium alginate

polyMG

polyM

polyG

Activity (U/mg)

1760.8 ± 15.8

2103.2 ± 21.3

1124.9 ± 8.2

2445.6 ± 12.7

Km (mM)

1.28 ± 0.28

0.92 ± 0.04

2.06 ± 0.17

0.69 ± 0.11

Vmax (nmol/s)

0.12 ± 0.03

0.15 ± 0.01

0.08 ± 0.01

0.18 ± 0.04

kcat (s−1)

2.22 ± 0.19

2.73 ± 0.23

1.48 ± 0.07

3.33 ± 0.21

kcat/Km (s−1/mM)

1.73 ± 0.32

2.97 ± 0.41

0.72 ± 0.17

4.83 ± 0.39

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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