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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Methanol fermentation increases the production of NAD(P)H-dependent chemicals in synthetic methylotrophic Escherichia coli

Fig. 2

The characterization of the synthetic E. coli. a Activity of MDH from Bacillus methanolicus MGA3 and an empty vector control in vivo were evaluated by analyzing the formaldehyde production in E. coli BL21(DE3) in M9 minimal media supplemented with 100 mM methanol. b Activity of HPS-PHI operon from Bacillus methanolicus MGA3 and empty vector control in vivo were assayed by analyzing the formaldehyde consumption in E. coli BL21(DE3) in M9 minimal media supplemented with 100 mM methanol. c The growths of the strains BL21/ΔfrmA-Mdh2-Hps-Phi, BL21/ΔfrmA-ACT-Mdh2-Hps-Phi, and BL21/ΔfrmA-NudF-Mdh2-Hps-Phi in M9 medium supplemented with 55 mM glucose and 100 mM methanol. d Methanol consumption by the strains BL21/ΔfrmA-Mdh2-Hps-Phi, BL21/ΔfrmA-ACT-Mdh2-Hps-Phi, and BL21/ΔfrmA-NudF-Mdh2-Hps-Phi in M9 medium supplemented with 55 mM glucose and 100 mM methanol. Error bars indicate standard error of the mean (n = 3)

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