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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Methanol fermentation increases the production of NAD(P)H-dependent chemicals in synthetic methylotrophic Escherichia coli

Fig. 5

Improved lysine production in synthetic methylotrophic E. coli. a Methylotrophic E. coli was engineered for the bioconversion of methanol to improve lysine production. The extra NADH from methanol consumption was engineered to generate NADPH for lysine production. b Lysine production in the strains BL21/ΔfrmA-ML and BL21/ΔfrmA-ML-POS5 cultivated with and without methanol. c Methanol consumption in the strains BL21/ΔfrmA-ML and BL21/ΔfrmA-ML-POS5. d The intracellular NADH pools in the strains BL21/ΔfrmA-ML and BL21/ΔfrmA-ML-POS5 cultivated with and without methanol. e The intracellular NADPH pools in the strains BL21/ΔfrmA-ML-POS5 cultivated with 55 mM glucose and 50 mM methanol. f Lysine production from 13C-methanol in the strains BL21/ΔfrmA-Mdh2-Hps-Phi, BL21/ΔfrmA-ML, and BL21/ΔfrmA-ML-POS5. Error bars indicate standard error of the mean (n = 3)

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