Fig. 3

a Biofilms of the WT and five mutants formed in 96-well plates under control conditions and with SNP treatment. b Photographs of biofilms formed on plastics by WT, ∆MAC1, ∆CTR1, +pMAC1, +pCTR1, and +p MAC1 CTR1. c Relative expression of MAC1 and downstream genes in ∆MAC1, ∆CTR1, +pMAC1, +pCTR1, and +pMAC1 CTR1, compared with WT. d Plate-wash tests of the WT, ∆MAC1, ∆CTR1, +pMAC1, +pCTR1, and +pMAC1 CTR1. Photos of pre- and post-washed strains were taken. The values are the means and standard deviations of three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05 by Student’s t-test