Skip to main content

Table 3 Intracellular copper and iron contents

From: Nitric oxide increases biofilm formation in Saccharomyces cerevisiae by activating the transcriptional factor Mac1p and thereby regulating the transmembrane protein Ctr1

  Strain Control p-value Treated with 200 μM SNP p-value
Copper (mg/kg dry cell weight) WT 3.35 0.0015** 4.57 0.0007***
∆MAC1 2.26 0.0064** 2.33 0.0398*
+pMAC1 4.02 0.0003*** 4.83 0.0229*
∆CTR1 1.69 0.014* 2.04 0.0061**
+pCTR1 3.87 0.0203* 4.91 0.0025**
Iron (mg/kg dry cell weight) WT 209.42 0.0315* 650.32 0.0573
∆MAC1 126.98 0.0019** 121.24 0.0028**
+pMAC1 517.40 0.0382* 804.91 0.0367*
∆CTR1 171.86 0.0006*** 435.01 0.0448*
+pCTR1 236.59 0.0197* 636.48 0.0732
  1. The copper and iron contents in WT and mutant strains biofilm cells were detected after 6 h of culture in the absence or the presence of SNP. The data are shown as milligrams of copper and iron per kg of cell dry weight. Each value is an average of three replicates
  2. *** p < 0.001, ** p < 0.01, * p < 0.05 by Student’s t-test