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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Proteomic study uncovers molecular principles of single-cell-level phenotypic heterogeneity in lipid storage of Nannochloropsis oceanica

Fig. 1

Workflow of FACS sorting and subsequent proteomic analysis of +N and –N N. oceanica subpopulations. After N. oceanica cells are stained with Nile Red for lipids, population heterogeneity can be detected by FACS-based cell sorting (+N P4 cells (blue diamonds); +N P3 cells (dark green diagonal lines); −N P4 cells (dark green chessboard); −N P3 cells (light green diagonal lines). Subsequent to sorting, cells are concentrated in 96 filter well plates, on which also cell lysis takes place. The extracted proteins are then digested by trypsin on the membrane. Data generated by mass spectrometry (MS) of these peptides are searched against a sequence database of N. oceanica proteins. Identified proteins are then quantified by LFQ algorithm of MaxQuant, to determine differences in proteome composition

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