Fig. 3
FACS sorting of –N N. oceanica cultures and proteomic abundance differences on carbon fixation pathway. a Cell density plot –N N. oceanica cells stained with Nile Red, the SSC (Sidescatter)-height channel informs about cell size and morphology, while the Nile Red Intensity reports the intensity of the FL2-Log-Height fluorescence channel. In this case, two subpopulations exist with one showing increased Nile Red fluorescence intensity in the FL2-Log-Height fluorescence channel. Percentage in upper left corner indicates percentage of depicted events from total. Percentages above subpopulations denote gated cell fraction from total. b Diagram of regulation of enzymes belonging to the carbon fixation pathway as well as to the glycolysis pathway between −N P3 and the −N P4 subpopulation. Significant regulation between subpopulations was calculated by a two-sample t test with a FDR cutoff by 0.05. Metabolites are highlighted by gray boxes and enzymes are highlighted by yellow boxes, color of the arrows shows upregulation or downregulation, while the numbers adjacent to the enzyme names are the regulation factors. Rb-1,5-P: ribulose-1,5-bisphosphate; G3P: 3-phospho glycerate; F-1,6-P: fructose-1,6-bisphosphate; TK: transketolase; GAP: glycerine aldehyde-3-phosphate; E-4-P: erythrose-4-phosphate; S-1,7-BP: sedoheptulose-1,7-bisphosphate; ShBP: sedoheptulose bisphosphatase; S-7-P: sedoheptulose-7-phosphate; X-5-P: xylulose-5-phosphate; RbPE: ribulose phosphate epimerase; ZC-desaturase: zeta-carotene desaturase; GG-reductase: geranylgeranyl-reductase; PSI: photosystem I; PSII: photosystem I; ALAD: aminolevulinic acid dehydratase; G-6-P: glucose-6-phosphate; DHAP: dihydroxyacetone-phosphate; TIM: triosephosphate isomerase; PEP: phosphoenolpyruvate; LDH: lactate dehydrogenase; PK: pyruvate kinase; TAG: triacylglycerol; LDSP: lipid droplet surface protein; ER: endoplasmic reticulum. CO2 fixation in Calvin cycle involving RbPE, ShBP and TK occurs in plastid, pigment biosynthesis involving ZC-desaturase and GC-reductase occurs in plastid. Energy production from carbon via glucose-6-phosphate to pyruvate and lactate involving TIM, PK and LDH occurs in cytosol. Storage and release of fatty acids in lipid droplet (yellow) involves LDSP and lipase