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Table 2 Transformation efficiencies of different C. saccharobutylicum mutants with unmethylated pMTL84151 plasmid

From: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

C. saccharobutylicum strain

Electroporation efficiencies

WT

0

ΔhsdR1

0

Ch1

58 ± 4

Ch2

255 ± 117

  1. Values are expressed in number of transformants per µg of unmethylated pMTL84151
  2. Mean values and standard deviations from two independent experiments are given
  3. 20 µg pMTL84151 was used in each experiment
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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