Table 6 Bacterial strains and plasmids used in this study

Bacterial strains

E. coli

 TOP10

Invitrogen

C. acetobutylicum

 ∆cac1502∆cac3535∆upp

∆CA_C 1502∆CA_C 3535∆CA_C 2879

[10]

 ∆cac1502 ∆cac3535∆upp ∆pSOL

∆CA_C 1502∆CA_C 3535∆CA_C 2879∆pSOL1

This study

 ∆cac1502 ∆cac3535 ∆upp ∆alsD

∆CA_C 1502∆CA_C 2879∆CA_C 3535∆CA_C 2967

This study

 ∆cac1502 ∆cac3535 ∆upp::cac0437*

∆CA_C 1502∆CA_C 2879∆CA_C 3535:: CA_C0437*

This study

 ∆cac1502 ∆cac3535 ∆upp::cac3368*

∆CA_C 1502∆CA_C 2879∆CA_C 3535:: CA_C3368*

This study

 ∆cac1502 ∆cac3535 ∆upp::spo0A*

∆CA_C 1502∆CA_C 2879∆CA_C 3535:: CA_C2071*

This study

C. saccharobutylicum

 ∆hsdR1

∆CLSA_RS02150

[5]

 Ch1 (∆hsdR1∆ hsdR2)

∆CLSA_RS02150 ∆CLSA_RS14125

[5]

 Ch2 (∆hsdR1∆ hsdR2 ∆hsdR3)

∆CLSA_RS02150 ∆CLSA_RS14125 ∆CLSA_RS04425

[5]

 ∆hsdR1∆ hsdR2∆ hsdR3 ∆upp

∆CLSA_RS02150 ∆CLSA_RS14125 ∆CLSA_RS04425∆CLSA_RS02460

This study

 ∆hsdR1∆ hsdR2∆ hsdR3 ∆upp, spo0A*

∆CLSA_RS02150 ∆CLSA_RS14125 ∆CLSA_RS04425∆CLSA_RS02460, CLSA_RS26780*

This study

Plasmids

 pAN1

Cm^r, ϕ3TI, p15A origin

[14]

 pUC18

Ap^r, colE1 origin

Fermentas

 pCR-BluntII-TOPO

Zeo ^r Km ^r

Invitrogen

 pCons2-1

Cm^r, repL

[10]

 pMTL84151

pCD6, Cm^R

[5]

 pCons::upp

MLS^r upp, repL

[10]

 pCR4-TOPO-Blunt

Ap ^r Km ^r Cm ^r

Invitrogen

 pCat-upp

Cm^r upp, colE1 origin

This study

 pEry-upp

MLS^r upp, p15A origin

This study

 pCat-upp-Dupp

Cm^r upp, upp deletion cassette for C. saccharobutylicum

This study

 pCat-upp-alsd

Cm^r upp, alsD deletion cassette for C. acetobutylicum

This study

 pCat-upp-spo0A*Csa

Cm^r upp, spo0A editing cassette for C. saccharobutylicum

This study

 pCat-upp-cac0437*

Cm^r upp, cac0437* editing cassette for C. acetobutylicum

This study

 pCat-upp-cac3368*

Cm^r upp, cac3368* editing cassette for C. acetobutylicum

This study