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Table 7 Oligonucleotides used for PCR amplifications

From: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

Primer name

Oligonucleotide sequence

pcat-Upp-F

AAAAAGGATCCGTGAGCAAAAGGCCAGCAAAAGGCC

pcat-Upp-R

AAAAAAGGATCCGTGAGCAAAAGGCCAGCAAAAGGCC

p15A-F

AAAAGGATCCTTAATAAGATGATCTTCTTGAGATCGTTTTGGT

p15A-R

AAAAGTCGACGCGCTAGCGGAGTGTATACTGGCTTA

eryUpp-F

AAAAGTCGACTCTACGACCAAAAGTATAAAACCTTTAAGAACTTTC

eryUpp-R

TATTTTACATTCTTTATTTTTTATTTTGTACCGAATAATCTATCTCCAGCATC

upp-Teradhe2-F

GATTATTCGGTACAAAATAAAAAATAAAGAATGTAAAATAGTCTTTGCTTCATTATATTAGC

teradhe2-R

AAAAGGATCCAAGATAAAAAACAAGAGTAAAATGTAAAATAGTCTATGTGC

Upp-Csa-1

ATTATGGATCCCCTGGAATGAAATATAGACATTATGCTCC

Upp-Csa-2

GTCCCAAATAATCTACTCATTTCATTATTCCTCCAAAACTTATATTATC

Upp-Csa-3

GGAATAATGAAATGAGTAGATTATTTGGGACTAAATAATCTGATGCAAG

Upp-Csa-4

ATAATGGATCCCGCACCTGCAAACGTAGTTGTAG

Upp-Check-F

ACGACCAGGTGGAATTAC

Upp-Check-R

CTTCCACATGGCCAACTC

Alsd-Cac-1

AAAATGATCACACCACATACAATTGCATATC

Alsd-Cac-2

GGTGAAGAAAAATGTAAGAGTATCCTAGAAGTGGTTTC

Alsd-Cac-3

TACTCTTACATTTTTCTTCACCTCAAACCAATTTATG

Alsd-Cac-4

AAAATGATCACCTTATTCATAATAATATGCCTCC

Alsd-Cac-F

TTAGAAACACCATTAGCACCTATAAAGGCT

Alsd-Cac-R

CGGTTAAACTTTTAAAAAAAGATAGCGATG

CAC0437_BAM_F

ATTGGATCCCTTGGCTTGAATGTATCAATGGAATTAAC

CAC0437_BAM_R

AATTGGATCCCCTTGTGAAGTTTGTGGTGGTAGC

CAC0437_EXT_F

CGATATGATCCCTATAGCACACG

CAC0437_EXT_R

CCTATGGGAGGGAAATCAACTTG

CAC0437_MAMA WT_F

GTAATGCTAAGACACAATTTATGGGGAC

CAC3368_BGLII_F

ATTAAGATCTTAGAAGTAGGCCCCATCTGCC

CAC3368_BGLII_R

ATTAAGATCTGGAGCGGTTATGAGAGAAAGACC

CAC3368_EXT_F

CCTGAGCTTATGGTACTCTGAAAGG

CAC3368_EXT_R

CATCTTGAGGAGTGTATGGAGATGC

CAC3368_MAMA WT_F

TATAGGAAGGTTTATAAAGAATATCCAAC

CAC3368_MAMA _R

TCCAGAGTTTGGCGACTACAT

Spo0A-Csa-1

TTTTGGATCCTCAAATAATTATTTAATGTTCCATTAGATAC

Spo0A-Csa-2

ATATCCTTTAATATGTGCAGGTACACTGATTTCATGAATGATGCTTGTAA

Spo0A-Csa-3

TTACAAGCATCATTCATGAAATCAGTGTACCTGCACATATTAAAGGATAT

Spo0A-Csa-4

TAATAAGGATCCTCAGATCCTAGATTGTTAGAGAAAACAGGA

Spo0A-Csa-F

TTTGAAATATTTTTTTCTTCTAAATAACTTG

Spo0A-Csa-R

AACTTCTAAATCAAACTTCTGTTGGTTCTAAAAG

Check_catp_F

AACTATTTATCAATTCCTGCAATTCGTTTAC

Check_catp_R

GGTATTTGAAAAAATTGATAAAAATAGTTG

pCat-Upp check_R

TCGCCACCTCTGACTTG

  1. Restriction sites used for the cassettes construction are underlined