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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Characterization of type I and type II diacylglycerol acyltransferases from the emerging model alga Chlorella zofingiensis reveals their functional complementarity and engineering potential

Fig. 2

Functional complementation of DGATs in the TAG-deficient yeast strain S. cerevisiae H1246. a BODIPY staining of H1246 cells expressing various C. zofingiensis DGATs. Left, bright field; right, fluorescent field. Green fluorescence indicates the BODIPY-bound lipid droplets. b TLC analysis of TAG. c Quantification of TAG, expressed as TAG/TFA ratio. d Fatty acid profile of TAG. EV (empty vector)-expressing H1246 strain was used as the negative control and C. reinhardtii DGTT1-expressing H1246 strain was used as the positive control (+). The data in (c) and (d) are expressed as mean ± SD (n = 3). Asterisks indicate the significant difference compared to EV (t-test, P < 0.01)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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