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Table 5 Final concentrations of potential inhibitory substances in SSF experiments with 1% w/w cellulose concentration with added hydrolysate from the first pretreatment stage

From: Two-stage steam explosion pretreatment of softwood with 2-naphthol as carbocation scavenger

2-Naphthol (mol/mol C9)

Concentration (g/l)

Acetic acid

HMF

Furfural

2-Naphthol

Washed solids

0 (control)

0.157

0.037

0.055

0

0.051

0.167

0.040

0.058

0.013

0.103

0.171

0.040

0.060

0.026

0.205

0.165

0.039

0.057

0.067

Filtered solids

0 (control)

0.279

0.237

0.076

0

0.051

0.269

0.213

0.078

0.017

0.103

0.272

0.226

0.073

0.027

0.205

0.268

0.217

0.073

0.072

Whole slurry

0 (control)

0.399

0.432

0.096

0

0.051

0.395

0.418

0.102

0.017

0.103

0.406

0.455

0.094

0.030

0.205

0.396

0.431

0.093

0.076

  1. 2-Naphthol was quantified after extraction of the whole fermentation slurry with chloroform