Skip to main content
Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization

Fig. 1

SDS-PAGE analysis of extracellular proteins (E) and cellulosomal (C) of C. thermocellum strains. The parent strain ∆pyrF produced both intact Cel48S and Cel9K proteins (black arrow). ∆pyrF::KBm also produced the wild-type Cel48S protein as ∆pyrF, but the Cel9K protein was rarely observed. An additional 150-kDa band was detected referring to the fusion protein Cel9K–BGL (red arrow). The intensities of the bands referring to Cel48S, Cel9K, and Cel9K–BGL were determined using the Quantity One software and were divided by that of the corresponding ScaA protein as previously described [33]. The obtained ratios shown below the lanes stand for the expression levels of the proteins. ND, not detected. Bands corresponding to known cellulosomal proteins are identified to the right of the Coomassie blue-stained gel. M, protein standards

Back to article page