Fig. 8

SPS saccharification under optimal or regular conditions without modification in both 100-mL anaerobic bottles (a) and a 10-L anaerobic fermenter (b). Regular conditions, ∆pyrF::CaBglA as the biocatalyst, inoculum size of 1%, GS-2 medium. Optimal conditions, ∆pyrF::KBm as the biocatalyst, inoculum size of 5%, mGS-2 medium, 150 U/g hemicellulase. 40 g/L SPS was supplemented as the substrate. The saccharification level was calculated by dividing the initial polysaccharides in the substrate (~ 0.89 g/g) with the amount of the obtained reducing sugar, which was determined by the DNS method. Two (10 L reaction) or three (100 mL reaction) parallels were prepared for each experiment